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ATCC mouse full length cd70 surface antigen

<t>CD70</t> expression correlates with survival in patients with solid tumors. A) (top) CD70 gene expression for different solid tumors compared with normal matched tissues. Sources: The University of Alabama at Birmingham Cancer data analysis (UALCAN) and The Cancer Genome Atlas program (TCGA). (Bottom panels) survival rates of patients diagnosed with renal carcinoma and lung cancer with low and high CD70 expression. Source: The Human Protein Atlas program. B-C) Confirmation of CD70 (target) expression on cancer cells – B) glioblastoma (GBM) and C) osteosarcoma (OS) models - and (bottom) transduction of chimeric antigen receptor (CAR) construct in B) C57BL/6 mice - derived T cells (CAR T Kr158B ) and C) in Balb/c mice - derived T cells (CAR T K7M2 ) as indicated by GFP reporter. On average, D) percentage of CD70 expression is 73% for glioblastoma and 99% for osteosarcoma models. E) Mean transduction of 66 % for CAR T Kr158B and 60% for CAR T K7M2 . The name of the cell lines and tumor models will be used interchangeably – GBM for Kr158B and OS for K7M2.

Mouse Full Length Cd70 Surface Antigen, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse full length cd70 surface antigen - by Bioz Stars, 2026-05

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1) Product Images from "Three-Dimensional Bioconjugated Liquid-Like Solid (LLS) Enhance Characterization of Solid Tumor - Chimeric Antigen Receptor T cell interactions"

Article Title: Three-Dimensional Bioconjugated Liquid-Like Solid (LLS) Enhance Characterization of Solid Tumor - Chimeric Antigen Receptor T cell interactions

Journal: bioRxiv

doi: 10.1101/2023.02.17.529033

CD70 expression correlates with survival in patients with solid tumors. A) (top) CD70 gene expression for different solid tumors compared with normal matched tissues. Sources: The University of Alabama at Birmingham Cancer data analysis (UALCAN) and The Cancer Genome Atlas program (TCGA). (Bottom panels) survival rates of patients diagnosed with renal carcinoma and lung cancer with low and high CD70 expression. Source: The Human Protein Atlas program. B-C) Confirmation of CD70 (target) expression on cancer cells – B) glioblastoma (GBM) and C) osteosarcoma (OS) models - and (bottom) transduction of chimeric antigen receptor (CAR) construct in B) C57BL/6 mice - derived T cells (CAR T Kr158B ) and C) in Balb/c mice - derived T cells (CAR T K7M2 ) as indicated by GFP reporter. On average, D) percentage of CD70 expression is 73% for glioblastoma and 99% for osteosarcoma models. E) Mean transduction of 66 % for CAR T Kr158B and 60% for CAR T K7M2 . The name of the cell lines and tumor models will be used interchangeably – GBM for Kr158B and OS for K7M2.

Figure Legend Snippet: CD70 expression correlates with survival in patients with solid tumors. A) (top) CD70 gene expression for different solid tumors compared with normal matched tissues. Sources: The University of Alabama at Birmingham Cancer data analysis (UALCAN) and The Cancer Genome Atlas program (TCGA). (Bottom panels) survival rates of patients diagnosed with renal carcinoma and lung cancer with low and high CD70 expression. Source: The Human Protein Atlas program. B-C) Confirmation of CD70 (target) expression on cancer cells – B) glioblastoma (GBM) and C) osteosarcoma (OS) models - and (bottom) transduction of chimeric antigen receptor (CAR) construct in B) C57BL/6 mice - derived T cells (CAR T Kr158B ) and C) in Balb/c mice - derived T cells (CAR T K7M2 ) as indicated by GFP reporter. On average, D) percentage of CD70 expression is 73% for glioblastoma and 99% for osteosarcoma models. E) Mean transduction of 66 % for CAR T Kr158B and 60% for CAR T K7M2 . The name of the cell lines and tumor models will be used interchangeably – GBM for Kr158B and OS for K7M2.

Techniques Used: Expressing, Gene Expression, Transduction, Construct, Derivative Assay

CAR T cell directed motion towards the target tumor. A) Representative confocal snapshots of CAR T cells infiltrating a GBM solid tumor in 3D. The panel shows CD70-specific CAR T cells (green) navigating through the supported COL1-LLS RhB microgels (red) and infiltrating the target GBM tumor (white). Blue arrows indicate paths of CAR T migration. B, D) mean velocity of CAR T cells cocultured with CD70 pos tumors and the WT control for B) GBM and D) OS tumor models. The number of tracks (n) is indicated on the plots. The number of biological replicates is N=2. An unpaired two-tailed Student’s t-test was performed. Statistical significance with p values was indicated on the plots. C, E) Quantifying tumor-infiltrating CAR T cells on average from 0-72h as a percent of total CAR T cells for C) GBM and E) OS tumors. The box plots display 25 th and 75 th percentiles, a line at the median, and a plus sign at the mean, from the minimum to the maximum observation. Unpaired two-tailed Student’s t-test was performed (n=234, N=3, statistical significance with p values were indicated on the plots). F) Top panel: Maximum intensity z projection (top panel) showing snapshots of CAR T – GBM tumor interaction at 0, 24, and 72h. Middle panel: the segmentation of CAR T with colors indicated by individual CAR T cells at each frame. The segmentation employed a deep learning-based method (as discussed in the method section). Bottom panel: maximum intensity projection of the segmented CAR T cell velocity tracks overtime. The velocity gradient was color-coded, showing accumulation of CAR T inside the tumor. The segmented cells were tracked using Linear Assignment Problem (LAP) tracker at the maximum frame-to-frame linking and allowable track segment gap closing of 150 μm (~ 3 cell diameter). G) Evidence of chemotaxis and upregulation in migratory pathways for CAR T cells co-cultured with their target tumors for GBM (top panel) and OS (bottom panel). Noticeably, evidence of immune-mediated cytotoxic function is demonstrated via IFNγ detection.

Figure Legend Snippet: CAR T cell directed motion towards the target tumor. A) Representative confocal snapshots of CAR T cells infiltrating a GBM solid tumor in 3D. The panel shows CD70-specific CAR T cells (green) navigating through the supported COL1-LLS RhB microgels (red) and infiltrating the target GBM tumor (white). Blue arrows indicate paths of CAR T migration. B, D) mean velocity of CAR T cells cocultured with CD70 pos tumors and the WT control for B) GBM and D) OS tumor models. The number of tracks (n) is indicated on the plots. The number of biological replicates is N=2. An unpaired two-tailed Student’s t-test was performed. Statistical significance with p values was indicated on the plots. C, E) Quantifying tumor-infiltrating CAR T cells on average from 0-72h as a percent of total CAR T cells for C) GBM and E) OS tumors. The box plots display 25 th and 75 th percentiles, a line at the median, and a plus sign at the mean, from the minimum to the maximum observation. Unpaired two-tailed Student’s t-test was performed (n=234, N=3, statistical significance with p values were indicated on the plots). F) Top panel: Maximum intensity z projection (top panel) showing snapshots of CAR T – GBM tumor interaction at 0, 24, and 72h. Middle panel: the segmentation of CAR T with colors indicated by individual CAR T cells at each frame. The segmentation employed a deep learning-based method (as discussed in the method section). Bottom panel: maximum intensity projection of the segmented CAR T cell velocity tracks overtime. The velocity gradient was color-coded, showing accumulation of CAR T inside the tumor. The segmented cells were tracked using Linear Assignment Problem (LAP) tracker at the maximum frame-to-frame linking and allowable track segment gap closing of 150 μm (~ 3 cell diameter). G) Evidence of chemotaxis and upregulation in migratory pathways for CAR T cells co-cultured with their target tumors for GBM (top panel) and OS (bottom panel). Noticeably, evidence of immune-mediated cytotoxic function is demonstrated via IFNγ detection.

Techniques Used: Migration, Control, Two Tailed Test, Chemotaxis Assay, Cell Culture

CAR T expansion, activation, and killing. A) Representative confocal timelapse images of the FITC channel (green) that show immune activation, expansion, and killing of the target tumors. The patterns of CAR T clustering and rapid expansion were observed in almost all conditions with efficient anti-tumor activity. White arrows locate CAR T cell clusters. B, C) The number of CAR T clusters rapidly increased during the first 24h and steadily maintained for more than 72h in B) GBM and C) OS models. D, E) Cluster size in all cocultures with target tumors steadily increased over time but not in the WT controls. F, G) Expansion of CAR T clusters revealed an inverse correlation with tumor size. (n=3 for all CD70 pos samples and n=2 for all WT samples). H, I) Endpoint flow cytometry data measuring CAR T expansion after 96h of CAR T – cancer cells coculture in the 2D assay for both tumor models. J, K) A comparison in IFNγ secretion (pg/mL) for 2D vs 3D for J) GBM and K) OS. The number of biological replicates is N=2. L) Top row: confocal 3D snapshots of GBM tumors after co-cultured with CAR T cells for 24h showing highly tortuous tumor margin of CD70 pos tumor as compared to the WT counterpart. Bottom row: cross-section view (z depth: 70 μm) of the sample on the top row exposing infiltrating CAR T cells within the tumor mass. M) Measurement of tumor tortuosity factors revealed more than 3-fold change for the CD70 pos tumors within the first 48h of coculture. The tumor tortuosity factor was calculated as a ratio between the perimeter of the tumor outline to the perimeter of a circle of the same pixel area. Data was obtained from GBM samples (n=3) at an initial E:T ratio of 1:2, and from osteosarcoma (K7M2) samples (n=3) at an initial E:T ratio of 1:1. Biological samples for each group (n= 3), and technical repetitions (n= 3) were performed.

Figure Legend Snippet: CAR T expansion, activation, and killing. A) Representative confocal timelapse images of the FITC channel (green) that show immune activation, expansion, and killing of the target tumors. The patterns of CAR T clustering and rapid expansion were observed in almost all conditions with efficient anti-tumor activity. White arrows locate CAR T cell clusters. B, C) The number of CAR T clusters rapidly increased during the first 24h and steadily maintained for more than 72h in B) GBM and C) OS models. D, E) Cluster size in all cocultures with target tumors steadily increased over time but not in the WT controls. F, G) Expansion of CAR T clusters revealed an inverse correlation with tumor size. (n=3 for all CD70 pos samples and n=2 for all WT samples). H, I) Endpoint flow cytometry data measuring CAR T expansion after 96h of CAR T – cancer cells coculture in the 2D assay for both tumor models. J, K) A comparison in IFNγ secretion (pg/mL) for 2D vs 3D for J) GBM and K) OS. The number of biological replicates is N=2. L) Top row: confocal 3D snapshots of GBM tumors after co-cultured with CAR T cells for 24h showing highly tortuous tumor margin of CD70 pos tumor as compared to the WT counterpart. Bottom row: cross-section view (z depth: 70 μm) of the sample on the top row exposing infiltrating CAR T cells within the tumor mass. M) Measurement of tumor tortuosity factors revealed more than 3-fold change for the CD70 pos tumors within the first 48h of coculture. The tumor tortuosity factor was calculated as a ratio between the perimeter of the tumor outline to the perimeter of a circle of the same pixel area. Data was obtained from GBM samples (n=3) at an initial E:T ratio of 1:2, and from osteosarcoma (K7M2) samples (n=3) at an initial E:T ratio of 1:1. Biological samples for each group (n= 3), and technical repetitions (n= 3) were performed.

Techniques Used: Activation Assay, Activity Assay, Flow Cytometry, Two-Dimensional Assay, Comparison, Cell Culture

Sensitivity of anti-tumor activity to various CAR T: cancer cell (E:T) ratios. A,B) confocal time-lapse images of CAR T – tumor co-culture in iVITA at different E:T ratios. C,D) show the digital image reconstruction of confocal data quantifying bulk tumor mass, migrating single cancer cells, immune cells, and immune cell clusters, and killing activity over time. A,C) GBM CD70 pos tumors and B,D) OS CD70 pos tumors were co-cultured with their respective CAR T cells at different concentrations corresponding to E:T of 1:4, 1:2, and 4:1 for the GBM model and E:T of 1:4, 1:1, and 4:1 for the OS model. At 72h and E:T= 4:1, almost 100% tumor elimination was observed in both models. E-H) CAR T expansion as a function of initial E:T seeding ratios. E,F) CAR T expansion on average in both models from 0-72h. For each seeding E:T, CAR T expansion at each time point was normalized to the CAR T at time 0, and the average was calculated for all frames. G,H) the number of CAR T clusters counted every 1.5h for 72h for each group. The box plots display 25th and 75th percentiles, a line at the median a plus sign at the mean, from the minimum to the maximum observation. I,J) Quantification of tumor size over time at the initial E:T= 1:4, 1:2, and 4:1 for I) GBM model and at E:T= 1:4, 1:1, and 4:1 for J) OS model. K,L) The E:T ratio dynamically changed over time. The CAR T expansion and tumor-killing were presented by the exponential increase of E:T ratios. M,N) tumor killing rates were calculated as derivatives from I) and J), respectively. Statistical analysis was performed using Ordinary One-Way ANOVA. (n=3 unless indicated otherwise, ** = p < 0.01, and **** = p < 0.0001).

Figure Legend Snippet: Sensitivity of anti-tumor activity to various CAR T: cancer cell (E:T) ratios. A,B) confocal time-lapse images of CAR T – tumor co-culture in iVITA at different E:T ratios. C,D) show the digital image reconstruction of confocal data quantifying bulk tumor mass, migrating single cancer cells, immune cells, and immune cell clusters, and killing activity over time. A,C) GBM CD70 pos tumors and B,D) OS CD70 pos tumors were co-cultured with their respective CAR T cells at different concentrations corresponding to E:T of 1:4, 1:2, and 4:1 for the GBM model and E:T of 1:4, 1:1, and 4:1 for the OS model. At 72h and E:T= 4:1, almost 100% tumor elimination was observed in both models. E-H) CAR T expansion as a function of initial E:T seeding ratios. E,F) CAR T expansion on average in both models from 0-72h. For each seeding E:T, CAR T expansion at each time point was normalized to the CAR T at time 0, and the average was calculated for all frames. G,H) the number of CAR T clusters counted every 1.5h for 72h for each group. The box plots display 25th and 75th percentiles, a line at the median a plus sign at the mean, from the minimum to the maximum observation. I,J) Quantification of tumor size over time at the initial E:T= 1:4, 1:2, and 4:1 for I) GBM model and at E:T= 1:4, 1:1, and 4:1 for J) OS model. K,L) The E:T ratio dynamically changed over time. The CAR T expansion and tumor-killing were presented by the exponential increase of E:T ratios. M,N) tumor killing rates were calculated as derivatives from I) and J), respectively. Statistical analysis was performed using Ordinary One-Way ANOVA. (n=3 unless indicated otherwise, ** = p < 0.01, and **** = p < 0.0001).

Techniques Used: Activity Assay, Co-Culture Assay, Cell Culture

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Journal: bioRxiv

Article Title: Three-Dimensional Bioconjugated Liquid-Like Solid (LLS) Enhance Characterization of Solid Tumor - Chimeric Antigen Receptor T cell interactions

doi: 10.1101/2023.02.17.529033

Figure Lengend Snippet: CD70 expression correlates with survival in patients with solid tumors. A) (top) CD70 gene expression for different solid tumors compared with normal matched tissues. Sources: The University of Alabama at Birmingham Cancer data analysis (UALCAN) and The Cancer Genome Atlas program (TCGA). (Bottom panels) survival rates of patients diagnosed with renal carcinoma and lung cancer with low and high CD70 expression. Source: The Human Protein Atlas program. B-C) Confirmation of CD70 (target) expression on cancer cells – B) glioblastoma (GBM) and C) osteosarcoma (OS) models - and (bottom) transduction of chimeric antigen receptor (CAR) construct in B) C57BL/6 mice - derived T cells (CAR T Kr158B ) and C) in Balb/c mice - derived T cells (CAR T K7M2 ) as indicated by GFP reporter. On average, D) percentage of CD70 expression is 73% for glioblastoma and 99% for osteosarcoma models. E) Mean transduction of 66 % for CAR T Kr158B and 60% for CAR T K7M2 . The name of the cell lines and tumor models will be used interchangeably – GBM for Kr158B and OS for K7M2.

Article Snippet: 293T cell line (ATCC, CRL-3216) was used for lentiviral plasmid production encoding for mouse full length CD70 surface antigen (GenBank: U78091.1 ).

Techniques: Expressing, Gene Expression, Transduction, Construct, Derivative Assay

Journal: bioRxiv

Article Title: Three-Dimensional Bioconjugated Liquid-Like Solid (LLS) Enhance Characterization of Solid Tumor - Chimeric Antigen Receptor T cell interactions

doi: 10.1101/2023.02.17.529033

Figure Lengend Snippet: CAR T cell directed motion towards the target tumor. A) Representative confocal snapshots of CAR T cells infiltrating a GBM solid tumor in 3D. The panel shows CD70-specific CAR T cells (green) navigating through the supported COL1-LLS RhB microgels (red) and infiltrating the target GBM tumor (white). Blue arrows indicate paths of CAR T migration. B, D) mean velocity of CAR T cells cocultured with CD70 pos tumors and the WT control for B) GBM and D) OS tumor models. The number of tracks (n) is indicated on the plots. The number of biological replicates is N=2. An unpaired two-tailed Student’s t-test was performed. Statistical significance with p values was indicated on the plots. C, E) Quantifying tumor-infiltrating CAR T cells on average from 0-72h as a percent of total CAR T cells for C) GBM and E) OS tumors. The box plots display 25 th and 75 th percentiles, a line at the median, and a plus sign at the mean, from the minimum to the maximum observation. Unpaired two-tailed Student’s t-test was performed (n=234, N=3, statistical significance with p values were indicated on the plots). F) Top panel: Maximum intensity z projection (top panel) showing snapshots of CAR T – GBM tumor interaction at 0, 24, and 72h. Middle panel: the segmentation of CAR T with colors indicated by individual CAR T cells at each frame. The segmentation employed a deep learning-based method (as discussed in the method section). Bottom panel: maximum intensity projection of the segmented CAR T cell velocity tracks overtime. The velocity gradient was color-coded, showing accumulation of CAR T inside the tumor. The segmented cells were tracked using Linear Assignment Problem (LAP) tracker at the maximum frame-to-frame linking and allowable track segment gap closing of 150 μm (~ 3 cell diameter). G) Evidence of chemotaxis and upregulation in migratory pathways for CAR T cells co-cultured with their target tumors for GBM (top panel) and OS (bottom panel). Noticeably, evidence of immune-mediated cytotoxic function is demonstrated via IFNγ detection.

Article Snippet: 293T cell line (ATCC, CRL-3216) was used for lentiviral plasmid production encoding for mouse full length CD70 surface antigen (GenBank: U78091.1 ).

Techniques: Migration, Control, Two Tailed Test, Chemotaxis Assay, Cell Culture

Journal: bioRxiv

Article Title: Three-Dimensional Bioconjugated Liquid-Like Solid (LLS) Enhance Characterization of Solid Tumor - Chimeric Antigen Receptor T cell interactions

doi: 10.1101/2023.02.17.529033

Figure Lengend Snippet: CAR T expansion, activation, and killing. A) Representative confocal timelapse images of the FITC channel (green) that show immune activation, expansion, and killing of the target tumors. The patterns of CAR T clustering and rapid expansion were observed in almost all conditions with efficient anti-tumor activity. White arrows locate CAR T cell clusters. B, C) The number of CAR T clusters rapidly increased during the first 24h and steadily maintained for more than 72h in B) GBM and C) OS models. D, E) Cluster size in all cocultures with target tumors steadily increased over time but not in the WT controls. F, G) Expansion of CAR T clusters revealed an inverse correlation with tumor size. (n=3 for all CD70 pos samples and n=2 for all WT samples). H, I) Endpoint flow cytometry data measuring CAR T expansion after 96h of CAR T – cancer cells coculture in the 2D assay for both tumor models. J, K) A comparison in IFNγ secretion (pg/mL) for 2D vs 3D for J) GBM and K) OS. The number of biological replicates is N=2. L) Top row: confocal 3D snapshots of GBM tumors after co-cultured with CAR T cells for 24h showing highly tortuous tumor margin of CD70 pos tumor as compared to the WT counterpart. Bottom row: cross-section view (z depth: 70 μm) of the sample on the top row exposing infiltrating CAR T cells within the tumor mass. M) Measurement of tumor tortuosity factors revealed more than 3-fold change for the CD70 pos tumors within the first 48h of coculture. The tumor tortuosity factor was calculated as a ratio between the perimeter of the tumor outline to the perimeter of a circle of the same pixel area. Data was obtained from GBM samples (n=3) at an initial E:T ratio of 1:2, and from osteosarcoma (K7M2) samples (n=3) at an initial E:T ratio of 1:1. Biological samples for each group (n= 3), and technical repetitions (n= 3) were performed.

Article Snippet: 293T cell line (ATCC, CRL-3216) was used for lentiviral plasmid production encoding for mouse full length CD70 surface antigen (GenBank: U78091.1 ).

Techniques: Activation Assay, Activity Assay, Flow Cytometry, Two-Dimensional Assay, Comparison, Cell Culture

Journal: bioRxiv

Article Title: Three-Dimensional Bioconjugated Liquid-Like Solid (LLS) Enhance Characterization of Solid Tumor - Chimeric Antigen Receptor T cell interactions

doi: 10.1101/2023.02.17.529033

Figure Lengend Snippet: Sensitivity of anti-tumor activity to various CAR T: cancer cell (E:T) ratios. A,B) confocal time-lapse images of CAR T – tumor co-culture in iVITA at different E:T ratios. C,D) show the digital image reconstruction of confocal data quantifying bulk tumor mass, migrating single cancer cells, immune cells, and immune cell clusters, and killing activity over time. A,C) GBM CD70 pos tumors and B,D) OS CD70 pos tumors were co-cultured with their respective CAR T cells at different concentrations corresponding to E:T of 1:4, 1:2, and 4:1 for the GBM model and E:T of 1:4, 1:1, and 4:1 for the OS model. At 72h and E:T= 4:1, almost 100% tumor elimination was observed in both models. E-H) CAR T expansion as a function of initial E:T seeding ratios. E,F) CAR T expansion on average in both models from 0-72h. For each seeding E:T, CAR T expansion at each time point was normalized to the CAR T at time 0, and the average was calculated for all frames. G,H) the number of CAR T clusters counted every 1.5h for 72h for each group. The box plots display 25th and 75th percentiles, a line at the median a plus sign at the mean, from the minimum to the maximum observation. I,J) Quantification of tumor size over time at the initial E:T= 1:4, 1:2, and 4:1 for I) GBM model and at E:T= 1:4, 1:1, and 4:1 for J) OS model. K,L) The E:T ratio dynamically changed over time. The CAR T expansion and tumor-killing were presented by the exponential increase of E:T ratios. M,N) tumor killing rates were calculated as derivatives from I) and J), respectively. Statistical analysis was performed using Ordinary One-Way ANOVA. (n=3 unless indicated otherwise, ** = p < 0.01, and **** = p < 0.0001).

Article Snippet: 293T cell line (ATCC, CRL-3216) was used for lentiviral plasmid production encoding for mouse full length CD70 surface antigen (GenBank: U78091.1 ).

Techniques: Activity Assay, Co-Culture Assay, Cell Culture

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